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High degree of colocalization of retinol and retinoic acid in mature suprabasal epidermal cells.
Retinoic acid (RA) is a vitamin A derivative that acts through its receptors (RA receptors) to influence many cellular processes, including skin development and differentiation. The cellular and molecular targets of RA are intimately associated with its receptor proteins, which are predominantly found in the suprabasal epidermis. However, the relationship between RA and the retinol-derived molecule, retinol, has not been determined in the developing epidermis. Here, the spatial distribution and distribution kinetics of RA in relation to retinol, in mouse skin, were determined by use of immunohistochemistry. In fetal and postnatal skin, anti-RA receptor protein 1 (RAR beta) and anti-RA receptor protein 2 (RAR alpha) antibodies stained cells in the suprabasal epidermis, with nuclear staining for RAR alpha and cytoplasmic staining for RAR beta. Staining by both RAR alpha and RAR beta antibodies was more intense than that obtained with RAR gamma antibodies that recognize only the latter receptor. During late fetal to early postnatal development, the fraction of cell-associated retinol was low, ranging from 15 to 27%, whereas the fraction of cell-associated RA was consistently higher, ranging from 42 to 68%. During the late fetal to early postnatal period, the fraction of cell-associated retinol was higher than that of RA, which was highest in newborn mice. Despite this difference, the fractions of cell-associated retinol and RA were strikingly similar, with the highest correlation for newborn mice. These observations support the hypothesis that there is a high degree of colocalization of ret
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