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Claveparaactivarwindows8singlelanguage . Clavepara is a movie review site that reviews certain movies in its own category.DNA typing and analysis on Arabidopsis thaliana mutations in the nuclear gene SAG102 for mitochondrial NADP-malate dehydrogenase.
In this report, we present the identification and chromosomal localization of the nuclear gene that codes for mitochondrial NADP-malate dehydrogenase in Arabidopsis thaliana. The SAG102 gene (At3g54660) is the third member of the gamma-subtype of mitochondrial NADP-malate dehydrogenases in Arabidopsis and maps on chromosome 3. It is monocistronic and the corresponding transcript is about 2.3 kb in length. In order to develop a PCR-based marker system, a segment of the SAG102 gene was sequenced, and then used to design PCR primers. By using these primers, a single PCR product of about 1 kb in length was amplified from all Arabidopsis DNA samples. This PCR product was sequenced, yielding about 700 bp of sequences from the Arabidopsis genome, which corresponded to the complete SAG102 gene. The gene was located on the 3rd chromosome by a previous cross between a SAG102 mutant and A. thaliana Landsberg erecta. Using seven other SAG102 mutants and their progeny, the gene was narrowed down to a region of about 50 kb on the short arm of the 3rd chromosome. Southern blots, using the PCR product as a probe, showed that this 50 kb segment contained both the SAG102 locus and the chloramphenicol acetyltransferase (CAT) gene, which was used as a marker in one of the mutants. Quantitative PCR was used to determine copy number variations in the mutants.//
// Copyright 2014 Marco Martiniello
//
// Licensed under the Apache License, Version 2.0 (the “License”);
// you may not use this file except in compliance with the License.
// You may obtain a copy of the License at
//
//
//
// Unless required by applicable law or agreed to in writing

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