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Apoptosis is a process of programmed cell death and this study was undertaken to investigate the timing of apoptosis during human endometrial development. Endometrial biopsies were obtained during the proliferative and mid-secretory (day 21 of the cycle) and the peri-implantation (5-7 days after luteinizing hormone (LH) injection) phases of the human endometrial cycle. Apoptosis was quantified by histomorphometric analysis of bcl-2 positive cells using in situ hybridization and an immunoreactive antibody. The data showed that the proportion of endometrial cells in the early secretory phase with positive bcl-2 staining was significantly greater than the mid-secretory phase. There was no significant difference in the number of apoptotic cells between proliferative and mid-secretory phase biopsies but significantly more apoptotic cells were found in the peri-implantation biopsies. There were no differences in the number of positively stained cells between proliferative and mid-secretory phase and between proliferative and peri-implantation biopsies. Thus, the data show that apoptosis occurs at the endometrial and zonal level during the human endometrial cycle and during early pregnancy. The data also demonstrate that apoptosis is associated with endometrial gland development and that there is regional variation in apoptotic cell numbers. Furthermore, the data suggest that endometrial apoptosis plays a role during early human endometrial development.Posted: October 22, 2009

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