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C4 gene expressions of patients with systemic lupus erythematosus and anti-C1q autoantibodies.
The objectives of this study were (1) to investigate the status of the complement system in systemic lupus erythematosus (SLE) patients and (2) to compare the effects of anti-C1q autoantibodies to those of other anti-C1q antibodies on the expressions of C1q, C4, C3, and the regulatory gene of C4 regulation (C4R). We studied the level of C1q, C4, C3, and the C4R gene expression in peripheral leukocytes and monocytes by using a semiquantitative reverse-transcription polymerase chain reaction (RT-PCR). The status of C4 expression and the C4/C3 ratio of the SLE patients were significantly lower than those of the normal controls. These data suggested that C4 might play an important role in the pathogenesis of SLE patients. The gene expressions of C4, C1q, and C3 in SLE patients with anti-C1q antibodies were lower in the peripheral leukocytes than those in patients without anti-C1q antibodies. The C4 expressions of SLE patients with anti-C1q antibodies were negatively correlated with the C4R gene expressions of SLE patients. The SLE patients with anti-C1q antibodies and low C4 gene expression might have a worse clinical manifestation. The C1q gene expression might be one of the factors which influence the clinical manifestation of SLE patients.

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