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Gene knockdown of vascular endothelial growth factor receptor-2 (VEGFR-2) in pancreatic cancer cells inhibits tumor growth by direct and indirect mechanisms. In this study, we investigated the effect of VEGFR-2 siRNA nanoparticle delivery to BxPC3 pancreatic cancer cells and their in vivo biodistribution. The effect of nanoparticle-mediated delivery of 3 different siRNAs on the expression of VEGFR-2 and its targeted protein, VE-cadherin, was investigated in vitro. The biodistribution of the nanoparticles in mice bearing BxPC3 pancreatic cancer xenografts was studied in vivo by fluorescence imaging. The effect of tumor growth inhibition after VEGFR-2 gene knockdown was demonstrated by the in vitro and in vivo assays. The results show that (1) uptake of the VEGFR-2 siRNA nanoparticles was receptor-mediated, (2) the three VEGFR-2 siRNAs showed a concentration-dependent knockdown of their respective target proteins, and (3) targeted delivery of the VEGFR-2 siRNAs to pancreatic cancer cells in vitro and in vivo reduced the expression of VEGFR-2 and the targeted protein, VE-cadherin, thus demonstrating the successful delivery of VEGFR-2 siRNAs. The biodistribution of the nanoparticles in vivo was studied by fluorescence imaging. The results show that the distribution of the nanoparticles was dependent on the size of the particles. Smaller particles were eliminated more rapidly from the circulation and were primarily found in the liver and spleen. More larger particles could be found in the lungs, suggesting their initial elimination from the blood stream.The aim of this study was to investigate the possible determinants of spatial variation in malaria in a non-endemic setting. The study was
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